Unnamed
(#49883313)
Level 1 Spiral
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Energy: 50/50
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Personal Style
Apparel
Skin
Scene
Measurements
Length
3.55 m
Wingspan
2.88 m
Weight
69.94 kg
Genetics
Gloom
Iridescent
Iridescent
Cyan
Shimmer
Shimmer
Sanddollar
Filigree
Filigree
Hatchday
Breed
Eye Type
Level 1 Spiral
EXP: 0 / 245
STR
5
AGI
9
DEF
5
QCK
8
INT
6
VIT
6
MND
6
Biography
(Poisonous Toridae)
It’s the 1st of the month. You’re trying to set up an experiment in which you will need 300x106 cells on the 20th this month.
You know that if you put 600,000 cells into a large tissue culture flask, you will get 10.5x106 cells after 4 days using 25mL of medium containing 10% FBS. This takes 10min per flask. If you use a small tissue culture flask, a seed of 200,000 cells will get you 6.4x106 after 4 days using 15mL of medium containing 10% FBS and taking only 6 minutes.
The longest you can leave cells growing is 4 days - any longer and they will die.
At the moment, you are maintaining this cell line by using a small Tissue Culture flask and harvesting them every 4 days and re-seeding the same flask with 200,000 cells. Today (the 1st) is the day these cells need to be harvested and re-seeded.
How would you plan this experiment, so that you have 300x106 or more cells ready to collect on the 20th this month, in the most cost-efficient (materials and time) way? How much cell medium and FBS will you need to prepare today to make sure you have enough to last you until the 20th?
It’s the 1st of the month. You’re trying to set up an experiment in which you will need 300x106 cells on the 20th this month.
You know that if you put 600,000 cells into a large tissue culture flask, you will get 10.5x106 cells after 4 days using 25mL of medium containing 10% FBS. This takes 10min per flask. If you use a small tissue culture flask, a seed of 200,000 cells will get you 6.4x106 after 4 days using 15mL of medium containing 10% FBS and taking only 6 minutes.
The longest you can leave cells growing is 4 days - any longer and they will die.
At the moment, you are maintaining this cell line by using a small Tissue Culture flask and harvesting them every 4 days and re-seeding the same flask with 200,000 cells. Today (the 1st) is the day these cells need to be harvested and re-seeded.
How would you plan this experiment, so that you have 300x106 or more cells ready to collect on the 20th this month, in the most cost-efficient (materials and time) way? How much cell medium and FBS will you need to prepare today to make sure you have enough to last you until the 20th?
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This dragon doesn't eat Seafood.
This dragon doesn't eat Plants.
Exalting Unnamed to the service of the Gladekeeper will remove them from your lair forever. They will leave behind a small sum of riches that they have accumulated. This action is irreversible.
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