Unnamed

(#49883313)
Level 1 Spiral
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Energy: 50/50
This dragon’s natural inborn element is Lightning.
Female Spiral
This dragon is hibernating.
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Personal Style

Apparel

Skin

Scene

Measurements

Length
3.55 m
Wingspan
2.88 m
Weight
69.94 kg

Genetics

Primary Gene
Gloom
Iridescent
Gloom
Iridescent
Secondary Gene
Cyan
Shimmer
Cyan
Shimmer
Tertiary Gene
Sanddollar
Filigree
Sanddollar
Filigree

Hatchday

Hatchday
Mar 04, 2019
(5 years)

Breed

Breed
Adult
Spiral

Eye Type

Special Eye Type
Lightning
Primal
Level 1 Spiral
EXP: 0 / 245
Scratch
Shred
STR
5
AGI
9
DEF
5
QCK
8
INT
6
VIT
6
MND
6

Lineage

Parents

Offspring

  • none

Biography

(Poisonous Toridae)

It’s the 1st of the month. You’re trying to set up an experiment in which you will need 300x106 cells on the 20th this month.

You know that if you put 600,000 cells into a large tissue culture flask, you will get 10.5x106 cells after 4 days using 25mL of medium containing 10% FBS. This takes 10min per flask. If you use a small tissue culture flask, a seed of 200,000 cells will get you 6.4x106 after 4 days using 15mL of medium containing 10% FBS and taking only 6 minutes.
The longest you can leave cells growing is 4 days - any longer and they will die.

At the moment, you are maintaining this cell line by using a small Tissue Culture flask and harvesting them every 4 days and re-seeding the same flask with 200,000 cells. Today (the 1st) is the day these cells need to be harvested and re-seeded.

How would you plan this experiment, so that you have 300x106 or more cells ready to collect on the 20th this month, in the most cost-efficient (materials and time) way? How much cell medium and FBS will you need to prepare today to make sure you have enough to last you until the 20th?

1-CD744-D7-498-E-4-FF1-A2-DB-5-F6616612104.jpg
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Exalting Unnamed to the service of the Gladekeeper will remove them from your lair forever. They will leave behind a small sum of riches that they have accumulated. This action is irreversible.

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